지방이식 for Dummies
지방이식 for Dummies
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Do you have a protocol or recommended antibodies to carry out ICC staining on my completely differentiated ALI cultures?
Blend gently before transferring cells to a conical tube made up of 10 mL of pre-warmed wash medium. Take note: Wash the pipette idea and cryovial With all the clean medium at least three occasions to help strengthen recovery.
Move cytometry can be utilized to evaluate the efficiency of differentiation protocols Together with the STEMdiff™ system, by investigating certain marker expression at Just about every stage from the differentiation procedure. It may also be used for other programs, which include cell sorting, immunophenotyping, and purity evaluation.
It is crucial to quench the dissociation reagent using FACS buffer or quit medium, by including at the least a similar or double the quantity of the dissociation reagent.
Carefully eliminate the supernatant without having disturbing the organoid pellet. Add 0.five mL of desired single-cell dissociation reagent for the tube. Distinct incubation pointers for various dissociation reagents are noted underneath:
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There are plenty of commercially readily available sources for primary airway epithelial cells. We have analyzed HAECs from two suppliers, Lonza and Epithelix — both equally have resulted in thriving ALI cultures.
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To evaluate the regional specificity of your 지방이식 smaller vs massive airway, you may perform the subsequent assays:
When planning tutorial RNAs, it is suggested to pick sequences that align Using the transcription start internet site on the gene of curiosity exactly where possible.
Complete cell counts employing an automated cell counter. Take note: Lower viability could be optimized by the size of collagenase or ACCUTASE™ incubation. It may also be optimized by reducing the amount of triturations.
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